Oral Presentation 24th Australian Conference on Microscopy and Microanalysis 2016

Flood gun parameters for charge control during imaging of insulating samples by Helium Ion Microscopy (HIM) (#82)

Peter J. Hines 1
  1. QUT, Brisbane, QLD, Australia

Abstract Summary

One of the advantages of Helium Ion Microscopy (HIM) is the ability to image insulating materials by using an electron flood gun for charge compensation. This paper examines the key parameters for successful imaging of a range of samples.

Introduction

Helium ion microscopes are becoming more widespread as tools for imaging and fabrication at the nano-scale. As in SEM, issues arise when imaging insulating samples (beam deflection, image distortion, brightness artifacts). As the sample charging is always positive (positive ions in; negative electrons out) the problems is simply solved, in principle, with an electron flood gun.

Stokes et al (1) developed a technique for dual-beam gallium FIBs where the electron beam is defocussed to the size of the milled pattern, and the current adjusted in proportion to the ion beam. While Fox et al (2), Joens et al (3) and Bidlack et al (4) demonstrate the effectiveness of the flood gun in imaging polymer, cell, and tooth samples respectively, little detail of the parameters is given. In practice, careful control of the imaging and flood gun parameters is required if imaging is to be successful at resolutions approaching the performance limit of the instrument.

Results

Investigating a range of charging samples some general parameter settings could be arrived at, along with a process for optimizing these parameters. While the flood gun output (~3 microamps) easily swamps the ion beam current (picoamperes) at lower magnifications, moving to fields of view of 10um or less requires careful optimisation of the beam and flood-gun parameters.

  1. DJ Stokes, F Morrissey, WR Knowles and AM Donald (2005) Some Charge Control Criteria for Focused Ion Beam Milling of Insulators and Biological Specimens using a Quanta 3D DualBeam ESEM. Microscopy and Microanalysis Volume 11 Supplement S02 August 2005, pp 804-805
  2. D Fox, Y Zhou and H Zhang (2015) Helium Ion Microscopy for Graphene Characterization and Modification in Nanotubes and Nanosheets: in Functionalization and Applications of Boron Nitride and Other Nanomaterials, Ying Chen (Ed) CRC Press.
  3. MS.Joens, C Huynh, JM Kasuboski, D Ferranti, YJ Sigal, F Zeitvogel, M Obst, CJ Burkhardt, KP Curran, SH Chalasani, LA. Stern, B Goetze, JAJ Fitzpatrick, (2013) Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution. Sci Rep. 2013; 3: 3514.
  4. FB Bidlack, C Huynh, J Marshman and B Goetze. (2014) Helium ion microscopy of enamel crystallites and extracellular tooth enamel matrix. Frontiers in Physiology 10 Oct 2014.